Dear,
I found the correctedReads number in featureDump.txt is a bit less than that in fastqc report. Here is the featuredumped.txt:
And here is the reads number from the same fastq using fastqc:
In my case, the length of read1 is 8 bp and they are all barcodes. I customized my_own_permit_list to include all the barcodes in reads1 and then generated the featureDump.txt by performing simpleaf quant using --explicit-pl my_own_permit_list.txt. so I suppose the correctedReads should be the same as that reported by fastqc. But it seems that correctedReads is less than the sequences number of reads1 fastq. What's the correctedReads ? where can find the documentation on featureDumped.txt ?
Dear,
I found the correctedReads number in featureDump.txt is a bit less than that in fastqc report. Here is the featuredumped.txt:
And here is the reads number from the same fastq using fastqc:
In my case, the length of read1 is 8 bp and they are all barcodes. I customized my_own_permit_list to include all the barcodes in reads1 and then generated the featureDump.txt by performing simpleaf quant using --explicit-pl my_own_permit_list.txt. so I suppose the correctedReads should be the same as that reported by fastqc. But it seems that correctedReads is less than the sequences number of reads1 fastq. What's the correctedReads ? where can find the documentation on featureDumped.txt ?