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FAQ

GFF format

  1. The software uses the regex: r'^(\S+)\t(\S+)\tCDS\t(\d+)\t+(\d+)\t+(\S+)\t+(\S+)\t+(\S+)\t+.*Parent=([\s\S]+?);' and r'^(\S+)\t(\S+)\tCDS\t(\d+)\t+(\d+)\t+(\S+)\t+(\S+)\t+(\S+)\t+.*Parent=([\S\s]+?)$' to parse the CDS feature lines in the gff file.
  2. The software uses the regex: r'^(\S+)\t(\S+)\t\S+\t(\d+)\t+(\d+)\t+(\S+)\t+(\S+)\t+(\S+)\t+.*ID=([\s\S]+?);Parent=([\s\S]+?);' and r'^(\S+)\t(\S+)\t\S+\t(\d+)\t+(\d+)\t+(\S+)\t+(\S+)\t+(\S+)\t+.*ID=([\s\S]+?);Parent=([\s\S]+?)$' to parse the mRNA feature lines in the gff file.

The annotation quality of non-chromosome segments in GFF files is poor, and gffread fails to run. Delete the annotation lines of non-chromosome sequences in GFF files

faq.ctg.error.png